Choose Enzymes Manually
Choose enzymes manually by name, or by editing an existing enzyme set or using search criteria.
Choose an Enzyme Set
To choose an existing enzyme set, click Enzymes → Choose Enzymes, then select the enzyme set name in the Choose from: list.
Choose an Enzyme by Name
To search within the chosen enzyme set, enter the enzyme name in the search box.
Alternatively, click on an enzyme name to select it.
Add to an Enzyme Set
To add the selected enzyme(s) to the Chosen Enzymes set, click the Add → button.
To add all of the enzymes from the original set to the Chosen Enzymes set, click the Add All → button.
Remove from an Enzyme Set
To remove the selected enzyme from the Chosen Enzymes set, click the Remove button or press the Delete key.
To remove all of the enzymes from the Chosen Enzymes set, click the Remove All button.
Choose Enzymes by Criteria
To choose enzymes based on criteria, click the Choose from check box. Enzymes that meet the selected criteria will be highlighted in the Choose from: list.
To specify the enzyme set from which the enzymes will be chosen, click the Choose from menu button.
To specify an enzyme set defined by where the chosen enzymes will cut, click the Enzymes cut menu button.
To specify the number of cut sites, type a number in the selected box. If not previously specified, the cut site region can be specified on the right.
To specify an enzyme set by the enzyme overhang type, click the Overhang menu button.
To specify the recognition sequence length, click the Recognition Sequence menu buttons.
To specify only enzymes with palindromic, uninterrupted, or nondegenerate recognition sequences, click the appropriate box.