User GuidesSnapGene User Guide Golden Gate AssemblySet the Enzymes for Golden Gate Assembly

Set the Enzymes for Golden Gate Assembly

How do change the default enzymes offered for use in Golden Gate Assembly?

The Default Enzyme list

By default, the SnapGene Golden Gate Assembly tool allows you to choose from a list of 9 Type IIS enzymes that are commonly used for Golden Gate Assembly. The default enzyme is BsaI.

Adjust the Golden Gate Enzymes List

Click Actions → Golden Gate Assembly → Golden Gate Settings... to show a list of all Golden Gate-compatible Type IIS enzymes in the SnapGene Enzyme database.


Use the check boxes to select the Enzymes you want to see as options for Golden Gate Assembly.

Use the dropdown menu to specify the default enzyme selected when the Golden Gate Assembly tool is started.

The selected enzymes will be available via the "Golden Gate Enzyme" dropdown menu when you run Actions → Golden Gate Assembly.