Golden Gate Assembly
How do I simulate Golden Gate assembly in SnapGene?
SnapGene does not currently have a dedicated tool for simulation of Golden Gate assembly.
You can use Actions → Restriction Cloning → Insert Multiple Fragments to simulate Golden Gate assembly of a vector and fragments with appropriate Type IIS enzyme sites (e.g. BsaI) at their termini.
For more information see the lesson Insert Multiple Restriction Fragments into a Plasmid.
If you would like to see development of a dedicated Golden Gate assembly tool for SnapGene then let us know by submitting an "improvement request" using our online support form.