Golden Gate Assembly

How do I simulate Golden Gate assembly in SnapGene?

SnapGene version 6.0.x and earlier does not have a dedicated tool for simulation of Golden Gate Assembly.

You can use Actions → Restriction Cloning → Insert Multiple Fragments to simulate Golden Gate Assembly of a vector and fragments with appropriate Type IIS enzyme sites (e.g. BsaI).

For more information see the lesson Insert Multiple Restriction Fragments into a Plasmid.

Coming Soon: SnapGene 6.1 is currently in beta testing and has a dedicated Golden Gate tool. Update to version 6.1 once it is released to access the new Golden Gate Assembly tools!