User GuidesSnapGene User Guide ProteinsMake a Protein Sequence from a DNA Sequence

Make a Protein Sequence from a DNA Sequence

How do I translate a coding sequence (CDS) feature or open reading frame (ORF) to a protein sequence?

Translate a CDS Feature

Open a DNA file with a Translated Feature (CDS). In Map view, click on the Feature to select the open reading frame (ORF) defined by the feature.

Translate the Feature

Click menu Actions → Make Protein....

Name the protein file.

Optional: Check "Merge segments" to create a protein with a single segment CDS annotation.

Click OK to create the new protein File.

Click File → Save to save the file to an appropriate location.

Translate a Predicted ORF

In this example we translate a predicted ORF generated due to an in-frame fusion of separate PMA1 and eGFP CDS features.

Shows ORFs and Set the Genetic Code for Translation

Open a DNA file, toggle the "Show Translations" button in the Side Toolbar to display predicted ORFs.

Right-click on the "Show Translations" button and choose Translation Options.

Use the drop down to set the appropriate Genetic code for translation of predicted ORFs. Adjust the length and start codon requirements for predicted ORFs if required. Click OK.

Click on the predicted ORF to select the region for translation.

Translate the Selected ORF

Click Actions → Make Protein....

Name the new protein file, then click OK to create a new protein File.

Click File → Save to save the protein file to an appropriate location.