User GuidesSnapGene User Guide Single Stranded SequencesCreate or Import a Single-Stranded DNA Sequence

Create or Import a Single-Stranded DNA Sequence

How do I create or import a single-stranded DNA sequence?

SnapGene 5.2 and later allow creation, import, and manipulation of single-stranded DNA (ssDNA) sequences.

Create a New Single-Stranded DNA Sequence

Click menu File → New DNA File to open the "New DNA File" dialog.

Type or paste a DNA sequence into the sequence field.

Set the Topology to "Single-Stranded", and select whether the sequence will be linear or circular.

Enter a name for the new sequence and click OK.


Import a Single-Stranded DNA Sequence from NCBI

Click menu File → Import → NCBI Sequences.

Enter the accession number(s) of the sequence(s) of interest, then click Import.

An NCBI GenBank record must have "ss-DNA" in the file LOCUS header to be imported by SnapGene as a single-stranded sequence. Note that not all NCBI records for single-stranded sequences contain the "ss-DNA" term. If a ssDNA sequence imports as dsDNA,  you can convert it — see  Convert Between Single-Stranded or Double-Stranded Formats.