Blast a DNA Sequence

How do I blast a SnapGene sequence against an NCBI database?

Import or Open a Sequence

Open a file, and in Map view or Sequence view, click Edit → Select All or manually select the region of the sequence you wish to "Blast".

Start Blast in SnapGene

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For a DNA comparison to a DNA database (blastn) click Tools → Blast Selected DNA → DNA Database....

For a translated DNA comparison to a protein database Blast (blastx) click Tools → Blast Selected DNA → Protein Database....

For a translated DNA comparison to a translated DNA database (tblastn) click Tools → Blast Selected DNA → Translated DNA Database....

SnapGene will copy the selected region and open the appropriate Blast page in the default web browser.

Note: If your combined sequence submission is large then SnapGene will notify you, copy the selected sequences to the clipboard, and request you paste the clipboard into "Query Sequence" field on the NCBI Blast web page.

Run NCBI Blast

Adjust NCBI Blast parameters if required, then click Blast to run the Blast search.

Import an NCBI Hit into Geneious

Use the Accession number (1) and/or range (2) to import the sequence into SnapGene, see Import a Sequence from NCBI.