Move the origin in a plasmid

How do I move the origin in a circular sequence?

Click View → Set Origin

In this example the origin (position +1) of the circular pmCherry-N1 plasmid is moved from between the ori and CMV promoter, to the first position in the mCherry CDS.

Note that position +1 is always displayed at the top of the circular map.

To move the origin (+1) position on a plasmid or circular sequence, in Sequence view, click to the left of the new +1 position and click View → Set Origin.  Confirm the correct position is selected then click OK to define the new origin.

The origin (+1) coordinate will now be set to the new position.